Add time:09/25/2019 Source:sciencedirect.com
A metabolic pathway for high level production of N-acetylglucosamine has been engineered in Escherichia coli by overexpressing E. coli glucosamine synthase (GlmS) and Saccharomyces cerevisiae glucosamine-6-phosphate acetyltransferase (GNA1). GlmS catalyzes the synthesis of glucosamine-6-phosphate from fructose-6-phosphate and glutamine. GNA1 converts glucosamine-6-phosphate into N-acetylglucosamine, which is dephosphorylated and secreted into the growth medium. In the present work, E. coli glucosamine-6-phosphate deaminase (NagB) was evaluated as an alternative to GlmS for the production of glucosamine and N-acetylglucosamine. NagB is a catabolic enzyme that converts glucosamine-6-phosphate to fructose-6-phosphate and ammonia. The reverse biosynthetic reaction forming glucosamine-6-phosphate is kinetically unfavorable. In a glmS deletion strain requiring glucosamine supplement to survive and grow, NagB overexpression resulted in the synthesis of glucosamine-6-phosphate. This supported cell growth, but little or no glucosamine accumulated in the medium. Overexpression of both NagB and GNA1 resulted in production of N-acetylglucosamine at levels comparable to strains overexpressing both GlmS and GNA1. This indicates that the overexpression of GNA1 played a critical role in determining the direction and efficiency of the reaction catalyzed by NagB. These data demonstrate that a catabolic enzyme can be utilized in a biosynthetic pathway by coupling with an efficient downstream reaction.
We also recommend Trading Suppliers and Manufacturers of N-ACETYLGLUCOSAMINE 6-SULFATE SODIUM SALT (cas 108321-79-5). Pls Click Website Link as below: cas 108321-79-5 suppliers
About|Contact|Cas|Product Name|Molecular|Country|Encyclopedia
Message|New Cas|MSDS|Service|Advertisement|CAS DataBase|Article Data|Manufacturers | Chemical Catalog
©2008 LookChem.com,License: ICP
NO.:Zhejiang16009103
complaints:service@lookchem.com Desktop View