Add time:09/05/2019         Source:sciencedirect.com

1. Analogues of U-U-U in which uridine residues were replaced by either 2-thiouridine, 4-thiouridine, 2,4-dithiouridine, or 2-thiocytidine were synthesized by enzymatic methods.2. The dinucleoside phosphates U-N and the trinucleoside diphosphates U-U-N were prepared by ribonuclease A-catalyzed transesterification.3. The dinucleoside U-N was elongated by using primer-dependent polynucleotide phosphorylase to yield U-N-U.4. For the synthesis of N-U-U the dinucleoside phosphate G-N, prepared with ribonuclease T1, was used as a primer in the polynucleotide phosphorylase-catalyzed reaction to yield G-N-U-U, from which the initial Gp was split off by ribonuclease T1.5. The coding properties of the newly synthesized trinucleoside diphosphates are reported. It follows that Ado·s2 Urd forms a strong base pair, whereas the base pairs Guo·s4 Urd, Guo·s2 Cyd, and Ado·s4 Urd are weak. No interaction between guanosine and 2-thiouridine or 2,4-dithiouridine could be detected in the binding assay.

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