Add time:09/08/2019 Source:sciencedirect.com
In order to investigate the mechanisms of uridine diphosphate glucose enzymic reactions, a number of analogues with modified uracyl residues were synthesized by the morpholidate method. 3-N-Methyl-uridine (I), 4-thiouridine (II), cytidine (III), 2-thiouridine (IV), iso-cytidine (V) and 6-azauridine (VI) diphosphate α-D-glucopyranoses were obtained.These modified nucleotides were prepared via 2′,3′-O-isopropylidine nucleosides the latter being obtained from uridine. Conditions are described for phosphorylation of nucleosides with dimorpholinochlorophosphate.
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