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  • The metabolism of [3-3H]oleanolic acid and its monoglycosides in cytoplasm and vacuole of protoplasts isolated from Calendula officinalis leaves
  • Add time:09/24/2019         Source:sciencedirect.com

    Isolated protoplasts from C. officinalis leaves were supplied with [3-3H]oleanolic acid, its 3-O-monoglucoside and 3-O-monoglucuronide. Transformations of these compounds into two series of oleanolic acid glycosides, i.e. glucosides (derivatives of 3-O-monoglucoside) and glucuronides (derivatives of 3-O-monoglucuronide) in the extravacuolar space and the vacuole were investigated. In the cytoplasm free oleanolic acid is glycosylated to both monoglycosides and to higher glycosides. Monoglycosides are partly hydrolysed to free oleanolic acid and partly glycosylated to higher derivatives. The vacuole contains the same radioactive compounds as the extravacuolar space. However, it seems most likely that these compounds are transported there from the sites of their synthesis in the cytoplasm.

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    Prev:Original articleBiosynthesis of oleanolic acid and its glycosides in Calendula officinalis suspension culture
    Next: The metabolism of [3−3H]oleanolic acid-3-O-mono-[14C]glucoside in isolated cells from Calendula officinalis leaves)

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