Add time:09/09/2019         Source:sciencedirect.com

The characteristics of a reaction involving pyrogallol and compounds related to glyceric acid in concentrated sulfuric acid were investigated. The reaction yielded a violet chromogen which exhibited an absorption maximum at 570 mμ. Among compounds reacting to form the chromogen, the following relative rates were observed: glycolaldehyde > glyceric acid > tartaric acid > 2-phosphoglyceric acid = 3-phosphoglyceric acid > 2,3-diphosphoglyceric acid.Optimal conditions for determination of monophosphoglycerates and 2,3-diphosphoglyceric acid are described. Specificity is increased by preheating the glycerates in sulfuric acid before addition of pyrogallol; contributions from the more rapidly reacting nonphosphorylated compounds are thereby reduced.Glyoxylic acid reacted with pyrogallol to produce a blue chromogen with an absorption maximum near 600 mμ.The colorimetric tests could be used for determinations of from 0.05 to 0.5 μmole of the acids under the conditions described.

We also recommend Trading Suppliers and Manufacturers of D(-)3-PHOSPHOGLYCERIC ACID CALCIUM (cas 108347-88-2). Pls Click Website Link as below: cas 108347-88-2 suppliers