Add time:09/24/2019 Source:sciencedirect.com
Two prenylated flavonoid derivatives, 5-hydroxy-4′-methoxy-2″,2″-dimethylpyrano-(7,8:6″,5″)flavanone (1) and 5,4′-dihydroxy-[2″-(1-hydroxy-1-methylethyl)dihydrofurano]-(7,8:5″,4″)flavanone (2), were isolated from an ethyl acetate-soluble extract of the leaves of Macaranga conifera using an in vitro activity-guided fractionation procedure based on the inhibition of cyclooxygenase-2. Also obtained were eight known compounds, 5,7-dihydroxy-4′-methoxy-8-(3-methylbut-2-enyl)flavanone (3), lonchocarpol A (4), sophoraflavanone B (5), 5,7-dihydroxy-4′-methoxy-8-(2-hydroxy-3-methylbut-3-enyl)flavanone (6), tomentosanol D (7), lupinifolinol (8), isolicoflavonol (9), and 20-epibryonolic acid (10). The structures of compounds 1 and 2 were determined using spectroscopic methods. All isolates were tested for their inhibitory effects against both cyclooxygenases-1 and -2, and selected compounds were evaluated in a mouse mammary organ culture assay.
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