Add time:09/09/2019 Source:sciencedirect.com
Rats maintained on a diet containing 0.2% DL-ethionine excreted an ultraviolet-absorbing compound in their urine that was not in the urine of rats fed the same diet without ethionine. The compound excreted was identified as S-adenosylethionine by chromatographic, spectrophometric, chemical, and isotopic means. Rats fed the diet containing ethionine for 10 weeks excreted about 5.6 μmoles of S-adenosylethionine per day. Rats injected with 613 μmoles (100 mg) of L-ethionine excreted about 7 μmoles of S-adenosylethionine in 48 h. S-adenosylethionine accounted for about 5% of the radioactivity excreted in the urine of ethionine-fed rats that were injected with [Et-I-14C]ethionine. Ethionine-fed rats excreted about 40% of the radioactivity recovered in the urine as a compound that was not found in the urine of control rats injected with [Et-I-14C]ethionine. This compound was identified as ethionine sulfoxide by paper chromatography and by conversion of the sulfoxide to ethionine with sodium metabisulite. The remainder of the radioactivity which accounted for 90% of the radioactivity excreted by control rats injected with [Et-I-14C]-ethionine was in an unidentified compound.
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