Add time:07/18/2019         Source:sciencedirect.com

Fatty acids (FA) are crucial for the maintenance of membrane fluidity and play a central role in metabolic energy storage. Polyunsaturated fatty acids play an essential ecological role since they are key parameters in the nutritional value of algae. Pesticide impacts on fatty acid profiles have been documented in marine microalgae, but remain understudied in freshwater diatoms. The aims of this study were to: 1) investigate the impact of diuron and S-metolachlor on “classical descriptors” (photosynthesis, growth rate, pigment contents, and on the expression levels of target genes in freshwater diatoms), 2) examine the impact of these pesticides on diatom fatty acid profiles and finally, 3) compare fatty acid profiles and “classical descriptor” responses in order to evaluate their complementarity and ecological role. To address this issue, the model freshwater diatom Gomphonema gracile was exposed during seven days to diuron and S-metolachlor at 10 μg.L−1.G. gracile was mostly composed of the following fatty acids: 20:5n3; 16:1; 16:0; 16:3n4; 14:0 and 20:4n6 and highly unsaturated fatty acids were overall the best represented fatty acid class. S-metolachlor decreased the growth rate and chlorophyll a content of G. gracile and induced the expression of cox1, nad5, d1 and cat genes, while no significant impacts were observed on photosynthesis and carotenoid content. In a more global way, S-metolachlor did not impact the fatty acid profiles of G. gracile. Diuron inhibited photosynthesis, growth rate, chlorophyll a content and induced cat and d1 gene expressions but no significant effect was observed on carotenoid content. Diuron decreased the percentage of highly unsaturated fatty acids but increased the percentage of monounsaturated fatty acids. These results demonstrated that fatty acids responded to diuron conversely to pigment content, suggesting that fatty acids can inform on energy content variation in diatoms subjected to herbicide stress.

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