Add time:09/06/2019         Source:sciencedirect.com

A carbon quantum dots (CQDs) based ratiometric nanosensor exhibiting dual emission fluorescence was developed. The spherical fluorescent carbon nanoparticles were obtained from lactose using a hydrothermal method. Under a single excitation at 355 nm they showed the typical blue emission at 417 nm and a red emission at 550 nm attributed to the coating effect of vitamin B12. The latter is explained by the fluorescence resonance energy transfer (FRET) and it consists of 61% of total emission. Upon the presence of carbofuran phenol 3-Keto, a phenolic metabolite of carbofuran, the red emission was quenched, resulting in a ratiometric fluorescence response in the blue range. This ratiometric nanosensor exhibited a good selectivity to carbofuran phenol 3-Keto. The ratio of I417/I550 linearly decreased with an increasing carbofuran phenol 3-Keto concentration in the range 9.8 μM–1.40 × 10−4 M. A detection limit for carbofuran phenol 3-Keto pesticide was 12.2 μM. The pesticide tested was extracted from soy sauce. Recovery values ranged from 98% to 114% with RSD values from 1.50% to 6.50%.

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