Add time:09/27/2019 Source:sciencedirect.com
Sucrose isomerase (SIase) has been used to produce PALATINOSE (cas 13718-94-0), a structural isomer of sucrose, which has many beneficial health properties, such as low-glycemic and low-insulinemic indices. A gene corresponding to SIase from Enterobacter sp. FMB-1 was expressed in Lactococcus lactis MG1363 using the P170 expression system. The autoinducible promoter (P170) and an optimized signal peptide (SP310mut2) were used to induce and secrete SIase in L. lactis. One-step Ni–NTA affinity chromatography and Western blot analysis demonstrated that SIase was successfully secreted to the culture supernatant, although 60% of the recombinant enzymes were retained inside the cells. The production of the recombinant SIase was highly correlated with pH (pH 6) and glucose concentration (30 g/L) of the medium. The extracellularly produced recombinant SIase was functionally active, effectively transforming 50 g/L sucrose to 36 g/L palatinose, with a conversion rate of 72% in the culture supernatant.
We also recommend Trading Suppliers and Manufacturers of PALATINOSE (cas 13718-94-0). Pls Click Website Link as below: cas 13718-94-0 suppliers
About|Contact|Cas|Product Name|Molecular|Country|Encyclopedia
Message|New Cas|MSDS|Service|Advertisement|CAS DataBase|Article Data|Manufacturers | Chemical Catalog
©2008 LookChem.com,License: ICP
NO.:Zhejiang16009103
complaints:service@lookchem.com Desktop View