Add time:07/16/2019         Source:sciencedirect.com

Actin polymerization has been studied in the absence of excess nucleotide. Using G-actin ATP monomers, it was shown that mechanical shearing stimulates ATP hydrolysis. The procedures used enabled the detection of differential effects of PHALLOIDIN (cas 17466-45-4) and tetramethylrhodamine-phalloidin, on the Pi-release step of the actin ATPase. It is concluded that tetramethylrhodamine, in contrast to phalloidin, accelerates Pi-release from actin filaments.

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