Add time:09/25/2019         Source:sciencedirect.com

We found that nitrite after protonation can react with amine radical on citric acid/ethylenediamine carbon nanodots (CA/EDA-CDs) to form nitrosamines, and fluorescence quenching of CA/EDA-CDs occurred during this process. Using the reaction mechanism a fluorescence quenching capillary analysis (FQCA) was developed. After optimized reaction conditions, the following results were obtained: the required concentration of CA/EDA-CDs was 12 mg/L, HCl concentration was 32 mmol/L, and the reaction conducted in room temperature for 20 min. Under optimized conditions, FQCA has a linear response in 20–500 μg/L in which RSD was less 4.5% (n = 11), the detection limit was 6.5 μg/L and the recovery was in 95–105%. The measured results were consistent with the national standard method. FQCA has been used for determining nitrite in foods and nature waters. The capillary in FQCA was used as the container for CA/EDA-CDs/NO2− reaction and NO2− determination, and realized trace-level analysis for micro-volume samples (<10 μL/time).

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