Add time:09/09/2019         Source:sciencedirect.com

The protoveratrine A and B complex can be quantitatively separated into its component alkaloids by partition chromatography over Celite® in which a mixture of pH 3.5 buffer and ethylene glycol is incorporated as immobile phase. Benzene elutes the protoveratrine A, while ethylene chloride elutes the protoveratrine B. Each alkaloid is uncontaminated by the other. A quantitative determination of protoveratrine is described, in which passage of a chloroform solution of the alkaloid over a partition chromatographic column containing cblorophenol red in the immobile phase removes quantities of dye proportional to the quantity of alkaloid.

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