Add time:09/26/2019         Source:sciencedirect.com

A quantitative GC method for conjugated linoleic acid (CLA) isomers of physiological significance (cis-9, trans-11 CLA and trans-10, cis-12 CLA) as non-esterified fatty acids (NEFA) or triacylglycerols (TAG) was developed. Furthermore, the effect of the internal standard addition point (sample or fat extract) was studied. Response linearity, recovery and precision assays, detection and quantification limits were determined. Linearity was demonstrated over a range from 0.1 to 10 μg/mL. When CLA isomers were present as NEFA, the recovery significantly decreased (P ≤ 0.05) from 76% to 27.1% (cis-9, trans-11 CLA) and 28.5% (trans-10, cis-12 CLA) when the standards were added to the fat extract or to the initial tissue, respectively. As an application, liver samples from hamsters fed a diet supplemented with both CLA isomers were analyzed. The CLA isomers in liver samples were detected with reasonable reproducibility.

We also recommend Trading Suppliers and Manufacturers of METHYL ESTER OF CLA (9-CIS, 11-TRANS) (cas 13058-52-1). Pls Click Website Link as below: cas 13058-52-1 suppliers