Add time:09/08/2019 Source:sciencedirect.com
The separation of 41 mono-, bis-, and tris-2,4-dinitrophenylhydrazones derived from simple saturated aldehydes and ketones, unsaturated aldehydes, di-, tri-, and hydroxycarbonyl compounds, and α-ketoacids by thin-layer chromatography is described. The cis- and trans-isomers of some of these compounds have been separated. The characteristic colours produced by different classes of 2,4-dinitrophenylhydrazones sprayed with ethanolamine are more lasting and more distinctive than those with alcoholic sodium or potassium hydroxide. A method for the chromatography of highly insoluble 2,4-dinitrophenylhydrazones is described. 2,4-Dinitrophenylhydrazones have been treated with acetylating agents, and with concentrated hydrochloric acid, directly on the thin-layer plates, and the chromatographic behaviour of the products has been studied. Methods for separating and identifying the components of complex mixtures of 2,4-dinitrophenylhydrazones are discussed.
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