Add time:09/08/2019         Source:sciencedirect.com

A sensitive and specific radioimmunoassay is described for plasma and urinary levels of [D-Ser(tBu)]6des-Gly-NH210 ethylamide (buserelin).No appreciable cross-reaction (<0.05%) was observed with LH-RH and its analogs other than buserelin fragments (1.6–45%). The sensitivity was 3 pg per tube. At buserelin concentrations of 125, 250 and 500 pg/ml, the intra- and inter-assay coefficients of variation were 7.9, 10.0 and 10.0%, and 19.0, 7.8 and 6.8% respectively. Recovery of buserelin added to plasma was quantitative (62.5 pg/ml, 101.6%; 125 pg/ml, 76.8% and 250 pg/ml, 63.4%). A dose of 5 μg buserelin injected subcutaneously into 5 normal male adults, reached a peak plasma level in 45 min (mean value 119.3±47.3 pg/ml) and remained detectable for at least 4 h. The half disappearance time was 118.8±26.0 min. Between 9 and 16% of the administered dose was excreted in the urine within 24 h. Buserelin could also be detected in the plasma after intranasal administration of doses of 150, 300 and 450 μg. There was a significant difference in the area under the curve (AUC) for plasma levels after subcutaneous injection of 5 μg and intranasal administration of 150 μg, but not between the AUC values after the three intranasal doses.These results indicate that this method for radioimmunoassay of buserelin is suitable for analyzing the pharmacokinetics and bioavailability of buserelin in man.

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