Add time:07/15/2019         Source:sciencedirect.com

Accurate and reliable methods are presented for the determination of enantiomeric excess values of glycidol (2,3-epoxy-1-propanol), glycidyl esters, solketal [2,2-dimethyl-4-(hydroxymethyl)-1,3-dioxolane], homologous 1,3-dioxolane alcohols and substituted primary propanols in biological samples. One method consists of derivatization of the samples with 2,3,4,6-tetra-O-acetyl-β-glucopyranosyl isothiocyanate, followed by separation of the resulting diastereomers on a non-chiral reversed-phase (C18) HPLC column. The second method uses direct injection of (aqueous) samples on to capillary GC columns coated with chiral stationary phases (2,3,6-tri-O-methyl-β- or a 2,3,6-tri-O-trifluoroacetyl-λ-cyclodextrin). The advantages and disadvantages of both methods are discussed.

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