Add time:09/24/2019 Source:sciencedirect.com
The reaction of the 3-benzyl ether of dimethyl marrianolate (I) with triphenylmethylsodium and then 3H2O produced I-3H in good yield. Treatment of I-3H with sodium in liquid ammonia (acyloin condensation) produced 16-ketoestradiol-15-3H. The latter was reduced by sodium amalgam to estriol-15-3H. The product, obtained in 14% overall isotope yield, was about 97% pure after column chromatography. In 2 metabolism studies conducted with human subjects, virtually no exchange of isotope with body water occured.
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