Add time:09/28/2019 Source:sciencedirect.com
Gas chromatography–mass spectrometry (GC–MS) and high performance liquid chromatography (HPLC) offer highly efficient and potentially sensitive separation and detection techniques. This work describes the quantification of Aminoglutethimide (cas 125-84-8) (AG) in nanocapsules suspension with both techniques. The analysis of different lots containing known concentrations of drug (1, 2, 3 and 4 mg ml−1) were used to investigate the quantitative capabilities of both chromatographic techniques. Both chromatographic methods were successful and on an analytical point of view the validations of aminoglutethimide dosing were suitable in both cases. In routine, the determination of the quality of nanocapsules suspension could be preferentially evaluated by difference between total AG concentration in suspension (evaluated by direct HPLC measure of the suspension diluted in acetonitrile) and free AG concentration (evaluated by direct HPLC measure of simple dilution of the supernatant).
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