Add time:09/25/2019 Source:sciencedirect.com
Steady-state parameters have been obtained for the tryptic hydrolysis of the ethyl esters of d-arginine and d-lysine. At acid pH the rates of hydrolysis are substantial. The stereospecificity ratio (kcatKm)l to (kcatKm)d is six times greater for the arginine ester than for the lysine ester. The stereospecificity ratios for the α-N-unsubstituted arginine and lysine esters are smaller by one to two orders of magnitude than those for the corresponding α-N-acylated derivatives. This decrease is due to a reduction in kcatKm for the l-isomer together with a proportionate increase in kcatKm for the d-isomer.
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