Add time:09/24/2019 Source:sciencedirect.com
An HPLC method using UV detection is proposed for the simultaneous determination of pseudophedrine hydrochloride, codeine phosphate, and triprolidine hydrochloride in liquid formulation. C18 column (250 mm × 4.0 mm) is used as the stationary phase with a mixture of methanol:acetate buffer:acetonitrile (85:5:10, v/v) as the mobile phase. The factors affecting column separation of the analytes were studied. The calibration graphs exhibited a linear concentration range of 0.06–1.0 mg/ml for pseudophedrine hydrochloride, 0.02–1.0 mg/ml for codeine phosphate, and 0.0025–1.0 mg/ml for triprolidine hydrochloride for a sample size of 5 μl with correlation coefficients of better than 0.999 for all active ingredients studied. The results demonstrate that this method is reliable, reproducible and suitable for routine use with analysis time of less than 4 min.
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