Add time:09/25/2019         Source:sciencedirect.com

Three new metabolites were obtained on incubation of androgenic steroid Mesterolone (cas 1424-00-6) (1) with Cunninghamella blakesleeana. These metabolites were identified as 1α-methyl-11β,14α,17β-trihydroxy-5α-androstan-3-one (2), 1α-methyl-7β,17β-dihydroxy-5α-androstan-3-one (3), and 1α-methyl,17β-hydroxy-5α-androstan-3,7-dione (4). During this study, hydroxylation at C-11, C-14, and C-15, and oxidation at C-7 of substrate 1 were observed. β-Hydroxylation at C-11 is a rather unique transformation by C. blakesleeana, as α-hydroxylation is reported to be catalyzed by most of the other microorganisms.

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