Add time:09/29/2019 Source:sciencedirect.com
A novel, simple, sensitive and selective spectrofluorimetric method was developed for the determination of Acetazolamide in pharmaceutical tablets and serum samples using photo probe Tb3+-ACAC. The Acetazolamide can remarkably quench the luminescence intensity of Tb3+-ACAC complex in DMSO at pH 6.8 and λex = 350 nm. The quenching of luminescence intensity of Tb3+-ACAC complex especially the electrical band at λem = 545 nm is used for the assessment of Acetazolamide in the pharmaceutical tablet and serum samples. The dynamic range found for the determination of Acetazolamide concentration is 4.49 × 10−9–1.28 × 10−7 mol L−1, and the limit of detection (LOD) and limit of quantification (LOQ) are (4.0 × 10−9 and 1.21 × 10−8) mol L−1, respectively.
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