Add time:10/01/2019 Source:sciencedirect.com
Urine and plasma samples of non-castrated, cryptorchid and castrated boars were analysed in order to establish the endogenous concentration of 19-nortestosterone. A combination of three different analytical techniques was used in this work. ELISA and gas chromatography were used for quantitation and gas chromatography-mass spectrometry for confirmation of positive samples. The results obtained confirm the endogenous origin of 19-nortestosterone in boars and give a natural range of concentration of about 1–300 ng ml−1 for urine and 1–15 ng ml−1 for plasma samples.
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