Add time:09/28/2019         Source:infona.pl

A simple, sensitive and selective method of high‐performance liquid chromatography with electrochemical detection (HPLC‐ECD) has been developed for simultaneous determination of BAVACHIN (cas 19879-32-4) and isobavachalcone in Fructus Psoraleae. At optimized conditions, bavachin and isobavachalcone could be well separated within 15 min at a detection potential of +0.80 V with 0.03 mol/L acetate buffer solution (pH 5.17)/acetonitrile (2:3, v/v) as the mobile phase. The relationships between peak areas and concentrations were linear from 8.26×10−7 to 1.21×10−4 mol/L for bavachin, and from 1.01×10−8 to 1.61×10−4 mol/L for isobavachalcone, respectively. The method offered excellent linearity with regression coefficient R2>0.995. The method presented detection limits (S/N=3) of 8.81×10−9 mol/L for bavachin and 1.17×10−10 mol/L for isobavachalcone. It indicates that the sensitivity of electrochemical detection is ten times higher than that of diode array detection (DAD). The mean recoveries around 98% with a relative standard deviation less than 3.1% for the two analytes have been obtained. The proposed separation and detection procedures were successfully applied to the simultaneous determination of bavachin and isobavachalcone in traditional Chinese medicine.

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