Add time:10/01/2019 Source:infona.pl
Streptomycin (STR) and Dihydrostreptomycin (cas 128-46-1) (DHSTR) are two of the most common aminoglycoside antibiotics used in veterinary medicine. The physicochemical properties of both substances, make their determination challenging. In the present study the development of methods based on ion-pair chromatography (IPC) and on hydrophilic interaction chromatography (HILIC), for the determination of the above mentioned aminoglycosides in the range of 100–1000μgL −1 is described. The two methods were validated according to EU requirements for residues in food. The recoveries for the IPC method were 69.3% and 56.5% of STR and DHSTR, respectively, and for HILIC method 85.5% and 72.3%, respectively. The intra- and inter-day precision, studied at 100, 200 and 300μgkg −1 levels in milk samples, gave %RSD≤13 for both methods. LOQs for the HILIC method were 14μgkg −1 for both analytes and for the IPC method were 109 and 31μgkg −1 , for STR and DHSTR, respectively. The sensitivity of the HILIC method is 80 and 210 times greater than that of the ICP method, for STR and DHSTR, respectively.
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