Add time:09/30/2019 Source:infona.pl
A sensitive capillary GC-MS method for the analysis of Dihydrostreptomycin (cas 128-46-1) has been developed. This method involves a new derivatisation procedure for dihydrostreptomycin with silylation of the hydroxyl groups with trimethylsilylimidazole and cyclisation of the guanidino groups with hexafluoroacetylacetone. The mass spectrum (electron impact ionisation) of the resulting derivative is given and interpreted. The derivatisation procedure has been optimised using multivariate and step-by-step optimisation procedures. Both approaches are explained, the respective results and their interpretation are discussed in detail. Comparison of both methods reveals, that the multivariate optimisation method is the more efficient and more reliable procedure.
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