Add time:09/26/2019 Source:infona.pl
This is an original work on catalytic performance of urease in organic synthesis which in one-pot dissociation of urea and condensation of the in situ generated ammonia with aldehydes, acetophenones, and malononitrile occurs in water to give 2-amino-3-cyanopyridines. Comparative experiments with ammonium salts supported the enzymatic specify of 0.01g/mL (50U/mg) of urease for bio-production of ammonia, while trace amount of heavy metal ions such as Pb2+, Hg2+, and Ag+ inhibit these specific reactions. The scalability and promiscuity of urease facilitate the applicability of the process for biotechnological organic reactions based on ammonia.
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