Add time:09/27/2019         Source:infona.pl

Matrix metalloproteinases (MMPs), the key enzymes in extracellular matrix degradation, seemed to increase in tumorigenesis, which has a relationship with metastasis and invasions. In this study, we investigated the MMP inhibitory effect of viridicatol and viridicatin (cas 129-24-8), two compounds extracted from shark-gill-derived fungus Penicilliumpolonicum AP2T1, on MMP-2 and -9 in vitro and in silico. The MTT assay showed that viridicatol and viridicatin had no cytotoxicity at a concentration of 50 µM on HT1080 human fibrosarcoma cells. In addition, the studies by gelatin zymography and Western blot analysis confirmed that the two compounds effectively inhibited the protein activities and expressions of MMP-2 and -9. Moreover, molecular docking studies simulated and clarified the interaction between compounds and MMPs, respectively. Furthermore, the effect of those two compounds on mitogen activated protein kinases (MAPKs) was also assessed by Western blot analysis. Collectively, these results suggested that viridicatol and viridicatin could be potential inhibitors of MMPs and P.polonicum may be a rich source of bioactive compounds with MMP inhibitory property.

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