Add time:09/28/2019 Source:infona.pl
A novel chemiluminescence (CL) flow system for two sulfite-containing drugs, namely, Menadione sodium bisulfite (cas 130-37-0) (MSB) and analgin is described. It is based on the weak chemiluminescence induced by the oxidation of sulfite group in drugs with dissolved oxygen in the presence of acidic Rh6G. Tween 80 surfactant micelles showed a strong enhancement effect on this weak chemiluminescence. For MSB analysis, online conversion of MSB in alkaline medium into sodium bisulfite was necessary, whereas analgin could be determined directly. The proposed method allowed the measurement of 0.05-50 μg ml - 1 MSB and 0.05-10 μg ml - 1 analgin. The limits of detection (3σ) were 0.01 μg ml - 1 MSB and 0.003 μg ml - 1 analgin. The method was applied satisfactorily to pharmaceutical preparations as well as biological fluids.
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