Add time:09/28/2019 Source:infona.pl
Single‐phase nanocrystalline biocompatible Zn‐doped A‐type carbonated hydroxyapatite (A‐cHAp) powder has been synthesized via mechanical alloying of a stoichiometric mixture of CaCO3, CaHPO4·2H2O and ZnO powders in open air at room temperature by 10 h of milling. The A‐type carbonation in HAp (A‐cHAp) is confirmed by Fourier transform IR analysis. The structural and microstructural parameters of the as‐milled powders are revealed by Rietveld powder structure refinement analysis and transmission electron microscopy. Zn substitution along with mechanical alloying causes partial amorphization of crystalline A‐cHAp, analogous to native bone mineral. Zn2+ cations substitute into the ninefold‐coordinated Ca2+ sites in the A‐cHAp unit cell. An assay test using MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] reveals a high percentage of cell viability and hence confirms the biocompatibility of the sample. The overall results indicate that the processed A‐cHAp has a chemical composition very close to that of natural biological apatite.
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