Add time:09/29/2019 Source:infona.pl
A novel anion‐exchange organic polymer monolithic column based on monomers N‐benzylquininium chloride and acrylamide were firstly prepared by in situ copolymerization for capillary electrochromatography. Moreover, N‐benzylquininium was firstly introduced as a strong anion‐exchange functional group. A relatively strong anodic EOF was obtained in the pH values from 4.0 to 9.0, which was in the same direction with the electrophoretic mobility of acid compounds. Hence, the anion‐exchange monolithic column was very suitable for the rapid separation of acid compounds. Eight acid compounds (2‐chlorobenzoic acid, mandelic acid, 4‐hydroxybenzoic acid, indole‐3‐acetic acid, 2‐aminoterephthalic acid, 3,5‐pyridinedicarboxylic acid, benzoic acid, and 4‐aminobenzoic acid) were successfully separated on the monolithic column. The highest column efficiency was 4.60 × 105 plates/m (theoretical plates, N) for 3,5‐pyridinedicarboxylic acid. The proposed monolithic column was characterized by SEM and FT‐IR. The RSDs of the acid compounds migration time for run‐to‐run, day‐to‐day, and column‐to‐column were all less than 5.0%.
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