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Berthelot's reaction (formation of indophenol-blue) is the basis of the colorimetry used by the great majority of participants, and their individual methods are examined in detail. Basic principles are discussed and procedures are compared. Various points of the reaction mechanism are examined in order to determine optimal ranges for reagent concentrations. The use of low-nutrient seawater as a calibration matrix is strongly recommended if errors such as those incurred by the use of demineralised water or artificial seawater are to be avoided. Sources of contamination are discussed.
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