Add time:09/30/2019 Source:infona.pl
Rheumatoid arthritis fibroblast‐like synoviocytes (RA‐FLSs), a pathological hallmark of rheumatoid arthritis (RA), exhibit the characteristics of tumor cells. The extracts of Cirsium japonicum var. ussuriense have been shown to possess antitumor and anti‐inflammatory activities. Our study aimed to investigate the effects of Pectolinarin (cas 134-33-8), a flavonoid compound isolated from C. japonicum var. ussuriense, on RA. Cell viability was evaluated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide assay. Apoptosis was determined by flow cytometry analysis and Western blot analysis of Bax and Bcl‐2 levels. Inflammation was assessed by detecting the expressions and secretion of interleukin (IL)‐6 and IL‐8 using quantitative real‐time polymerase chain reaction and enzyme‐linked immunosorbent assay, respectively. The production of nitric oxide (NO) and prostaglandin E2 (PGE2) was also measured. The effects of pectolinarin on the phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) pathway were examined by Western blot. We found that pectolinarin significantly inhibited cell viability at 24 and 48 hours in a dose‐dependently manner in RA‐FLSs. Pectolinarin reduced the apoptotic rate, increased Bax level, and decreased Bcl‐2 level in RA‐FLSs. Pectolinarin inhibited the messenger RNA expression and secretion of IL‐6 and IL‐8, as well as the production of PGE2 and NO in RA‐FLSs. Furthermore, pectolinarin inactivated the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) pathway in RA‐FLSs. Activation of the PI3K/Akt pathway by 740Y‐P impaired the effects of pectolinarin on cell viability, apoptosis, and inflammation in RA‐FLSs. In conclusion, pectolinarin suppressed cell proliferation and inflammatory response and induced apoptosis in RA‐FLSs via inactivation of the PI3K/Akt pathway.
We also recommend Trading Suppliers and Manufacturers of Pectolinarin (cas 134-33-8). Pls Click Website Link as below: cas 134-33-8 suppliers
About|Contact|Cas|Product Name|Molecular|Country|Encyclopedia
Message|New Cas|MSDS|Service|Advertisement|CAS DataBase|Article Data|Manufacturers | Chemical Catalog
©2008 LookChem.com,License: ICP
NO.:Zhejiang16009103
complaints:service@lookchem.com Desktop View