Add time:10/01/2019         Source:infona.pl

A quantitative high-performance liquid chromatographic method in which amiloride is separated from methylclothiazide on a C 18 column with detection at 286 nm was developed with the aid of the ‘window diagram’ technique of Laub and Purnell. The effect of simultaneously varying the pH and methanol to water ratio in the mobile phase were studied to optimize the separation. The method was applied to the quantitative analysis of Lometazid tablets. The powdered tablets were extracted with methanol, containing phenacetin as the internal standard, and assayed by comparison of peak heights after liquid chromatography.

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