Add time:09/29/2019 Source:infona.pl
THIONIN (cas 135-59-1)s have a considerable potential as antimicrobial compounds although their application may be restricted by metal ion-based inhibition of membrane permeabilizing activity. We previously reported the properties associated with the proposed mechanism of metal ion-based inhibition of β-purothionin. In this study, we investigated the effects of metal ions on α-hordothionin which differs from β-purothionin by eight out of 45 residues. Three of the differing residues are thought to be involved in the mechanism of metal ion-based inhibition in β-purothionin. The structure and dynamics of α-hordothionin were explored using unconstrained molecular dynamics (MD) simulations in explicit water as a function of metal ions. Although the global fold is almost identical to that of β-purothionin, α-hordothionin displays reduced fluctuating motions. Moreover, α-hordothionin is more resistant to the presence of metal ions than β-purothionin. Mg +2 ions do not affect α-hordothionin, whereas K + ions induce perturbations in the α2 helix, modify dynamics and electrostatic properties. Nevertheless, these changes are considerably smaller than those in β-purothionin. The proposed mechanism of metal ion-based inhibition involves the hydrogen bonding network of Arg5–Arg30–Gly27, which regulates dynamic unfolding of the α2 C-end which is similar to β-purothionin response. The key residues responsible for the increased resistance for α-hordothionin are Gly27 and Gly42 which replace Asn27 and Asp42 involved into the mechanism of metal ion-based inhibition in β-purothionin. Comparison of MD simulations of α-hordothionin with β-purothionin reveals dynamic properties which we believe are intrinsic properties of thionins with four disulphide bonds.
We also recommend Trading Suppliers and Manufacturers of THIONIN (cas 135-59-1). Pls Click Website Link as below: cas 135-59-1 suppliers
About|Contact|Cas|Product Name|Molecular|Country|Encyclopedia
Message|New Cas|MSDS|Service|Advertisement|CAS DataBase|Article Data|Manufacturers | Chemical Catalog
©2008 LookChem.com,License: ICP
NO.:Zhejiang16009103
complaints:service@lookchem.com Desktop View