Add time:10/01/2019         Source:infona.pl

BACKGROUND Trans‐cinnamic acid (t‐CA) and its derivatives have been shown to be antioxidant and antibacterial, and can be used in food additives and flavors. However, a traditional t‐CA production via chemical synthesis with a condensation reaction may not be suitable for its application in the pharmaceutical and food fields as it is not a ‘green’ route. This study investigated the bioconversion of l‐phenylalanine (l‐phe) to t‐CA using engineered whole‐cell Escherichia coli BL21(DE3) expressing phenylalanine ammonia‐lyase (PAL, EC 4.3.1.5) as a biocatalyst. To prevent product inhibition, an in situ reaction and separation of t‐CA was performed using a pH‐controlled biotransformation strategy.RESULTS A coupling reaction and separation system based on pH adjustment was proposed for improving the bioproduction of t‐CA. Using this process, 39.61 g L−1 t‐CA was bioproduced from 40 g L−1 of l‐phe in 8 h and a high purity (>98%) of t‐CA product was achieved directly without additional purification.CONCLUSION The bioprocess developed in this study is a highly efficient method for the bioproduction of t‐CA and, therefore, offers a promising alternative route for t‐CA production. © 2019 Society of Chemical Industry

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