Add time:09/28/2019 Source:infona.pl
Capillary electrophoresis method for identification and simultaneous determination of procaine, dihydrostreptomycin and penicillin G, present in multiantibiotic veterinary preparations, was elaborated. The influence of pH (5.0-9.75) and concentration of disodium tetraborate decahydrate in running buffers (0.02-0.1M) as well as temperatures (25-40 o C) on separation efficacy were analyzed. For quantitative analysis, 0.08M borate buffer (pH 8.0) at 35 o C and 15kV were chosen. Method was validated, selectivity, precision, linearity, LOD, LOQ, accuracy and specificity of capillary zone electrophoresis (CZE) were evaluated.
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