Add time:07/16/2019         Source:sciencedirect.com

An automated method for the determination of cellulase activity has been developed. The enzyme incubation with the cellulose substrate is stopped at alkaline pH, after which the reaction mixture is dialyzed. The concentration of reducing sugar in the dialyzer recipient is determined by means of the colorimetric ferricyanide-molybdoarsenic acid reaction. In this reaction the ferrocyanide formed by the ferricyanide reaction is coupled with molybdoarsenic acid during formation of a molybdenum-blue color. An optimization of the analytical conditions and the reagent concentrations has been carried out. The result of the automation is an analysis with high precision (mean = 36.4; SD = 0.7%) and accuracy as well as high rate of analysis. The sensitivity is 5 mg of glucose/liter.

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