Add time:07/20/2019         Source:sciencedirect.com

A rapid and sensitive method for the determination of naproxen and salicylate in serum is presented. The employed strategy combines solid-phase extraction on a reverse-phase membrane with spectrofluorimetry. Solid-phase extraction under optimum pH conditions makes NX to be retained over the solid surface (where it is directly determined by a fluorimetric technique). Salicylate passes through the disk and is also fluorimetrically determined, but in solution. The linear calibration ranges for NX in the membrane and salicylate in solution were 0.014–0.250 and 0.010–0.250 μg ml−1, respectively. The lowest value, in each case, is the corresponding limit of quantitation. The performance of the method is demonstrated with the successful determination of both drugs in spiked and real human serum samples.

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