Add time:07/17/2019         Source:sciencedirect.com

8-(6-Aminohexyl)-amino-AMP and 8-(6-aminohexyl)-amino-NAD(H) are synthesized from AMP and NAD+, respectively, in a two-step procedure via the 8-Br-adenine nucleotides. The AMP derivatives and the NAD+ derivatives possess some biological activity as substrates in the adenylate kinase and as coenzymes in the alcohol and lactate dehydrogenase systems, respectively.The synthesized derivatives may be coupled to Sepharose by cyanogen bromide activation. The covalently attached ligands show strong affinities for various dehydrogenases. Examples of the selective purification of several dehydrogenases using the new affinity columns are presented.

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