Add time:07/16/2019 Source:sciencedirect.com
Reaction products of NAD with 3-propiolactone at pH 6 showed that 3-propiolactone not only alkylates at the N-1 position of the adenine ring of NAD, but also reacts with other positions on NAD and its derivatives. Modifications other than the N-1 alkylation lowered the yield of N6-(2-carboxyethyl)-NAD. To improve the selectivity of the reaction of NAD with 3-propiolactone, reaction conditions were examined. The reaction at pH 3.5 gave pure 1-(2-carboxyethyl)-NAD in a yield of 73%, and the overall yield of N6-(2-carboxyethyl)-NAD was about 50%.
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