Add time:07/14/2019 Source:sciencedirect.com
The aim of this study is to ascertain the in vitro bioavailablity of pesticides that regulate and inhibit the growth of insects – flufenoxuron, lufenuron, pyriproxyfen and fenoxycarb – in grapes grown under good agricultural practice (GAP), while respecting the pre-harvest intervals (PHI) for critical conditions (CAP), in the most unfavorable conditions. The bioavailability of wines obtained from grapes in each assay and in standard solutions is also studied in order to establish matrix-related differences. Human gastric digestion, intestinal digestion and absorption were imitated. Porcine pepsin, porcine pancreatin, bile salts and semipermeable cellulose dialysis tubing were used.The analysis of the residues of the insecticides studied was performed by extraction with the QuEChERS method, and determination was with HPLC-MS.In all cases it was observed that the pesticides can be ordered according to their dialyzation capacity: fenoxycarb > pyriproxyfen > lufenuron > flufenoxuron. The different matrices can also be ordered according to the matrix effect they impose on the dialysis: grape > wine > standards. The highest percentages of dialyzation for grape and wine matrices are achieved for fenoxycarb (3.27%) and pyriproxifen (2.04%) in wine.
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