Add time:07/21/2019         Source:sciencedirect.com

The aim of this study was to prove experimentally that an inexpensive high-performance liquid chromatography system for routine control, operating at moderate pressures as predicted by the theoretical optimization model of Martin, Eon and Guiochon, is feasible.Two improved types of Spherosil (cas 11130-14-6), XOA 600 and XOA 800, lead to better selectivities and efficiencies (800 plates/cm), short columns (5–10 cm), that can be operated at moderate pressures (10–30 bar) and reasonable analysis times (5–15 min).The low pressure drops are due mainly to: the spherical shape of the particles, an average particle diameter of 5–7 μm, a narrow particle-size distribution (90% of the fraction between the limits d̄p ± 2 μm), a high specific surface area (600 or 800 m2/g) (the larger the surface area the shorter is the column and consequently the lower the pressure drop) and the linear velocity of the mobile phase fixed at the minimum of the Van Deemter curve.Numerous examples of separations confirm the potential of this new trend in high-performance liquid chromatography.

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