Add time:07/18/2019         Source:sciencedirect.com

3,4-Dimethylbenzoic acid and 3,5-dimethylbenzoic acid were both oxidised by 4-methylbenzoate (p-toluate)-grown cells of Rhodococcus rhodochrous N75 via the ortho-pathway through the intermediates 3,4- and 3,5-dimethylcatechol, respectively. Owing to the formation of the two novel dead-end metabolites, 4-carboxymethyl-2,3-dimethylbut-2-en-1,4-olide and 4-carboxymethyl-2,4-dimethylbut-2-en-1,4-olide from these substrates, 3,4- and 3,5-dimethylbenzoate did not serve as growth substrates for the strain.

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