Add time:07/18/2019         Source:sciencedirect.com

We devised a sensitive and simple method to determine alpha-methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine, using gas chromatography–mass spectrometry (GC–MS). AMT and 5MeO-DIPT were extracted using an Extrelut® column with an internal standard, bupivacaine, followed by derivatization with acetic anhydride. The derivatized extract was used for GC–MS analysis of EI-SIM mode. The calibration curves of AMT and 5MeO-DIPT were linear in the concentration range from 10 to 750 ng/ml in both blood and urine samples. The method detection limit (MDL) of AMT and 5MeO-DIPT were 1 ng/ml each in whole blood and 5 ng/ml each in urine. This method should be most useful to accurately determine the presence of these drugs in blood and urine in clinical and forensic cases.

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