• Name

  NILE RED

• EINECS 230-966-0
• CAS No. 7385-67-3
• Article Data11
• CAS DataBase
• Density 1.23g/cm³
• Solubility

Not hazardous according to Directive 67/548/EEC.

Toxicity data
(The meaning of any abbre

• Melting Point 203-205 °C(lit.)
  
• Formula C20H18 N2 O2
• Boiling Point 484.7°C at 760 mmHg
• Molecular Weight 318.375
• Flash Point 246.9°C
• Transport Information
• Appearance solid
• Safety 279110
• Risk Codes
• Molecular Structure
• Hazard Symbols
• Synonyms 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one;A 84655; Nile Blue A oxazone; Nile red; Phenoxazone 9
• PSA 46.34000
• LogP 4.29220

Synthetic route

90-15-3

α-naphthol

25953-06-4

4-nitroso-3-hydroxy-N,N-diethylaniline hydrochloride

7385-67-3

Nile Red

Conditions

Conditions	Yield
In toluene at 120℃; for 12h; Inert atmosphere;	79.1%
In N,N-dimethyl-formamide at 150℃; for 4h; Inert atmosphere;	21%

2-O-(2,4-dinitrobenzenesulfonyl)-9-(diethylamino)-5H-benzo[a]phenoxazin-5-one

7385-67-3

Nile Red

Conditions

Conditions	Yield
With L-Cysteine In dimethyl sulfoxide at 20℃; for 0.166667h; pH=7.4; Reagent/catalyst;

132-86-5

1,3-dihydroxynaphthalene

120-22-9

N,N-diethyl-4-nitrosoaniline

7385-67-3

Nile Red

Conditions

Conditions	Yield
In N,N-dimethyl-formamide at 80℃; for 16h;	80%

90-15-3

α-naphthol

91-68-9

3-diethylaminophenol

7385-67-3

Nile Red

Conditions

Conditions	Yield
Stage #1: 3-diethylaminophenol With hydrogenchloride; sodium nitrite In water at 0 - 5℃; for 4h; Stage #2: α-naphthol In N,N-dimethyl-formamide at 70℃; for 8h;	505 mg
Stage #1: 3-diethylaminophenol With hydrogenchloride; sodium nitrite In ethanol; water at 0 - 5℃; for 4h; Stage #2: α-naphthol In ethanol; water; N,N-dimethyl-formamide at 80℃; for 4h;

91-68-9

3-diethylaminophenol

7385-67-3

Nile Red

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: sodium nitrite; hydrogenchloride / 4 h / 0 °C 2: toluene / 12 h / 120 °C / Inert atmosphere View Scheme
Multi-step reaction with 2 steps 1: sodium nitrite; hydrogenchloride / water / 1.5 h / 0 °C 2: N,N-dimethyl-formamide / 4 h / 150 °C / Inert atmosphere View Scheme

90-15-3

α-naphthol

72375-53-2

2-(4-nitrophenyl)azo-5-diethylaminophenol

7385-67-3

Nile Red

Conditions

Conditions	Yield
In N,N-dimethyl-formamide at 150℃;	15%

25953-06-4

4-nitroso-3-hydroxy-N,N-diethylaniline hydrochloride

135-19-3

β-naphthol

7385-67-3

Nile Red

Conditions

Conditions	Yield
In N,N-dimethyl-formamide for 4h; Reflux;	24%

2-O-(2,4-dinitrobenzenesulfonyl)-9-(diethylamino)-5H-benzo[a]phenoxazin-5-one

A

3165-76-2

S-(2,4-Dinitrophenyl)-cystein

B

7385-67-3

Nile Red

Conditions

Conditions	Yield
With L-Cysteine

2-amino-7-diethylamino-3.4-benzo-phenoxazinylium sulfate

2-amino-7-diethylamino-3.4-benzo-phenoxazinylium sulfate

7385-67-3

Nile Red

Conditions

Conditions	Yield
With sulfuric acid; xylene

7-diethylamino-2-benzylamino-3.4-benzo-phenoxazinylium chloride

7-diethylamino-2-benzylamino-3.4-benzo-phenoxazinylium chloride

7385-67-3

Nile Red

Conditions

Conditions	Yield
With hydrogenchloride; xylene

90-15-3

α-naphthol

hydrochloride of 6-nitroso-3-diethylamino-phenol

hydrochloride of 6-nitroso-3-diethylamino-phenol

7385-67-3

Nile Red

Conditions

Conditions	Yield
With acetic acid

83-72-7

2-Hydroxy-1,4-naphthoquinone

25912-14-5

2-amino-5-(diethylamino)phenol dihydrochloride

A

7385-67-3

Nile Red

B

2-(4-diethylamino-2-hydroxy-anilino)-[1,4]naphthoquinone

Conditions

Conditions	Yield
With ethanol

...Expand

SNile Red

7385-67-3

Nile Red

Conditions

Conditions	Yield
With oxygen Quantum yield; UV-irradiation;

Nile red Chemical Properties

Nile red Safety Profile