1093881-86-7Relevant articles and documents
Azide conjugatable and pH responsive near-infrared fluorescent imaging probes
Murtagh, Julie,Frimannsson, Daniel O.,O'Shea, Donal F.
, p. 5386 - 5389 (2009)
"Chemical Equation Presented" The synthesis and photophysical characteristics of a pH responsive near-Infrared fluorescence Imaging probe Is described. A key feature is the ability to conjugate the probe by an alkyne-azide cycloaddition reaction and its r
Comparative triad of routes to an alkyne-BF2 azadipyrromethene near-infrared fluorochrome
Wu, Dan,O'Shea, Donal F.
, p. 4468 - 4472 (2017)
A series of three inter-related synthetic routes to a water soluble alkyne substituted BF2-azadipyrromethene fluorochrome is described. Each route allows for the introduction of the required reactive alkynyl group and water solubilizing propane-sulfonic acid through either more advanced pyrrole intermediates or through their earlier synthetic precursors. The advantages and disadvantages of each route lie in the number of synthetic steps required versus the formation of undesired azadipyrromethene by-products. Upon its synthesis, the alkyne-bearing fluorochrome was shown to efficiently participate in CuACC reactions with a series of azido substrates in water at room temperature and 37 °C. NIR-photophysical properties of the fluorochrome and its conjugates were similar, showing strong potential for in vitro and in vivo imaging applications.
Near-infrared fluorescent probe for evaluating the acetylcholinesterase effect in the aging process and dietary restrictionviafluorescence imaging
He, Na,Yu, Lei,Xu, Minghua,Huang, Yan,Wang, Xiaoyan,Chen, Lingxin,Yue, Shouwei
supporting information, p. 2623 - 2630 (2021/04/02)
Dietary restriction (DR), as a natural intervention, not only benefits the neuroendocrine system, but also has an antiaging action. Acetylcholinesterase (AChE) is one of the most important bioactive substances and plays a major part in choline changes in the aging process. Thus, we aim to evaluate the effect of DR on AChE in the brains of aging animals. In this study, we synthesize a NIR fluorescent probe BD-AChE for the real-time andin situmonitoring of AChE level changes in living cells and living mice, notably in brains.In situvisualization with BD-AChE verified a decrease in the AchE level in the brains of mice aging models. Evidently, the prepared probe has the excellent capability of measuring AChE variation in the brains of aging mice with DRviaNIR fluorescence bioimaging, indicating that long-term DR can effectively affect AChE levels in the brain. The attenuation of AChE level in the brain of aging mice after DR could be helpful in infering the advantageous impact of DR on age-related neurodegenerative disease, as a better treatment alternative in the future.
A convenient method for multicolour labelling of proteins with BODIPY fluorophores via tyrosine residues
Cheng, Miffy H.Y.,Savoie, Huguette,Bryden, Francesca,Boyle, Ross W.
, p. 1260 - 1267 (2017/08/16)
Fluorescence is an essential imaging modality for labelling and visualising cells and sub-cellular structures. Multicolour labelling is especially challenging due to differences in physicochemical and photophysical behaviour of structurally unrelated fluorophores in the heterogeneous environments found in sub-cellular compartments. Herein, we report the conjugation of three azide-bearing BODIPYs with similar core structures but widely different emission wavelengths (green, red and NIR) to tyrosine residues of a model globular protein (BSA) via a common linking methodology. The resulting BODIPY-BSA conjugates have demonstrated multi-wavelength fluorescence emission for biological applications. Fluorescence imaging was performed in HeLa cells through live cell confocal microscopy imaging, with good intracellular location visualisation observed.
