1353016-71-3 Usage
Description
DBCO-NHS Ester, also known as Dibenzylcyclooctyne NHS Ester, is a popular amine-reactive click chemistry reagent derived from DBCO (Dibenzylcyclooctyne). It is characterized by its ability to modify amine-containing molecules in organic solvents, with limited solubility in aqueous media. DBCO-NHS Ester reacts with primary amines, such as the side chain of lysine residues or aminosilane-coated surfaces, at neutral or slightly basic pH to form covalent bonds. Its low molecular weight adds minimal spacer to modified molecules, making it suitable for various applications in different industries.
Uses
Used in Bioconjugation:
DBCO-NHS Ester is used as a bioconjugation agent for the modification of amine-containing molecules. Its ability to form covalent bonds with primary amines allows for the creation of stable and specific bioconjugates, which are essential in various research and diagnostic applications.
Used in Cu-free Click Chemistry:
DBCO-NHS Ester is used as a Cu-free click chemistry reagent, enabling strain-promoted copper-free azide-alkyne cycloaddition reactions. This feature makes it a valuable tool in the synthesis of various bioconjugates and macromolecules without the need for toxic copper catalysts.
Used in DNA-Assisted Protein Analysis:
In the field of molecular biology, DBCO-NHS Ester is used as a reagent for DNA-assisted protein analysis, such as proximity ligation and extension assays. Its reactivity with azide-bearing biomolecules allows for the efficient and specific labeling of target proteins.
Used in Intracellular Imaging:
DBCO-NHS Ester is employed in intracellular imaging applications, where its ability to form covalent bonds with cellular components enables the visualization and tracking of specific biomolecules within cells.
Used in Drug Delivery Systems:
In the pharmaceutical industry, DBCO-NHS Ester is utilized in the development of drug delivery systems. Its photocleavable nitrobenzyloxyl group allows for the efficient release of conjugated molecules under UV light, enabling controlled drug release and improved therapeutic outcomes.
Used in Chemical Synthesis:
DBCO-NHS Ester is also used in chemical synthesis, particularly in the preparation of complex organic molecules and macromolecules. Its reactivity with primary amines and its low molecular weight make it a versatile building block for various chemical structures.
Check Digit Verification of cas no
The CAS Registry Mumber 1353016-71-3 includes 10 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 7 digits, 1,3,5,3,0,1 and 6 respectively; the second part has 2 digits, 7 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 1353016-71:
(9*1)+(8*3)+(7*5)+(6*3)+(5*0)+(4*1)+(3*6)+(2*7)+(1*1)=123
123 % 10 = 3
So 1353016-71-3 is a valid CAS Registry Number.
1353016-71-3Relevant articles and documents
ANTIBODY-DRUG CONJUGATES CONTAINING AN ANTI-MESOTHELIN ANTIBODY AND USES THEREOF
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Paragraph 0133-0134; 0161-0164, (2021/12/29)
The present disclosure provides an immunoconjugate includes an antibody comprising an antigen-binding fragment that specifically binds to an epitope in mesothelin, N-glycan binding domain and an N-glycan; a linker linking to the N-glycan; and a payload A and a payload B conjugated to the linker, respectively; wherein the payload A and the payload B are the same or different. A pharmaceutical composition comprises the immunoconjugate and a method for treating cancer are also provided in the disclosure.
NOVEL LINKERS FOR CONJUGATION OF CELL-BINDING MOLECULES
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Paragraph 0050, (2015/10/28)
Cell binding agent-drug conjugates comprising hydrophilic linkers, and methods of using such linkers and conjugates are provided.
Strain-promoted copper-free "click" chemistry for 18F radiolabeling of bombesin
Campbell-Verduyn, Lachlan S.,Mirfeizi, Leila,Schoonen, Anne K.,Dierckx, Rudi A.,Elsinga, Philip H.,Feringa, Ben L.
, p. 11117 - 11120 (2012/02/02)
Click for PET: The GRP-receptor-specific peptide bombesin, which is often used for nuclear imaging of tumors, can be labeled with 18F in a mild and rapid manner by using a copper-free azide-alkyne "click" reaction. A range of azides can be used to provide peptides with different hydrophobicities. The resulting 18F radiopharmaceutical tracers (see scheme) maintain their high affinity for the targeted receptor in vitro in human prostate cancer cells. Copyright
