16982-21-1Relevant articles and documents
1,2,4-Triazine-Modified 2′-Deoxyuridine Triphosphate for Efficient Bioorthogonal Fluorescent Labeling of DNA
Peewasan, Krisana,Wagenknecht, Hans-Achim
, p. 1473 - 1476 (2017)
In order to establish the Diels–Alder reaction with inverse electron demand for postsynthetic DNA modification, a 1,2,4-triazine-modified 2′-deoxyuridine triphosphate was synthesized. The bioorthogonally reactive 1,2,4-triazine group was attached at the 5-position of 2′-deoxyuridine by a flexible alkyl linker to facilitate its acceptance by DNA polymerases. The screening of four DNA polymerases showed successful primer extensions, using a mixture of dATP, dGTP, dCTP, and the modified 2′-deoxyuridine triphosphate, by using KOD XL or Vent polymerase. The triazine moiety was stable under the conditions of primer extension, which was evidenced by labeling with a BCN-modified rhodamine at room temperature in yields of up to 82 %. Two or three modified bases could be incorporated in quantitative yields when the modification sites were separated by three base pairs. These results establish the 1,2,4-triazene group as a bioorthogonally reactive moiety in DNA, thereby replacing the problematic 1,2,4,5-tetrazine for postsynthetic labeling by the Diels–Alder reaction with inverse electron demand.
METHODS AND COMPOSITIONS FOR INHIBITING CNKSR1
-
Paragraph 0154, (2014/07/07)
Embodiments include compositions and methods of inhibiting CNKSR1 and methods of identifying inhibitors of CNKSR1.
PAR4 AGONIST PEPTIDES
-
Page/Page column, (2013/11/06)
The present invention provides PAR4 agonist peptides. These peptides are useful for developing robust PAR4 receptor assays.
